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Neuropathology Research

RPA Neuropathology performs basic and translational research into human neurological disease. As a specialist neuro-diagnostics unit we focus on tissue-based research for enhanced diagnostics as well as basic disease pathophysiology.

Our Research Team

Associate Professor Michael Buckland
BSc (Med), MBBS, PhD, FRCPA, FFSc (RCPA)
Head of Department
Clinical Associate Professor, Discipline of Pathology, University of Sydney
Director, Australian Sports Brain Bank
Director, Neuropathology Tumour Tissue Bank
Co-Director, MS Australia Brain Bank
Dr Laveniya Satgunaseelan
MBBS, FRCPA
Staff Specialist Neuropathologist
Andrew Affleck
BSc
Neuropathology Brain Bank Manager
Dr Kim Alexander
BMedSc(Hon) PhD
Head of Brain Cancer Research, Chris O'Brien Lifehouse
Honorary Senior Research Officer, Sydney Local Health District
Manager, Sydney Brain Tumour Bank
Honorary Senior Research Fellow, University of Sydney
Danielle Davies
BSc
Biobanking Scientific Officer
Dr Csilla- Ágota T'zesi
PhD
Postdoctoral Scientist, Brainstorm Research
Dr Susannah Hallal
BSc, PhD
Postdoctoral Brain Cancer Research Fellow, Chris O'Brien Lifehouse
Honorary Research Officer, Sydney Local Health District

Selected Grants

Amount awarded Grant and project details
$100,000 NSW Health Biospecimen Collection Grant, 2021-2024
Sydney Brain Tumour Bank and GlioNET Observational study
Investigators: Varikatt W, Alexander-Kaufman K,  Buckland M
$60,000 BrainStorm Brain Cancer Research Stipend, 2020-2023
Developing a blood test for brain cancer
Investigators: Kaufman K

Our Publications

2022

Hallal S, Tűzesi Á, Grau GE, Buckland ME, Alexander KL. Understanding the extracellular vesicle surface for clinical molecular biology. J Extracell Vesicles. 2022 Oct;11(10):e12260. doi: 10.1002/jev2.12260. PMID: 36239734; PMCID: PMC9563386.
Buckland ME, Affleck AJ, Pearce AJ, Suter CM. Chronic Traumatic Encephalopathy as a Preventable Environmental Disease. Front Neurol. 2022 Jun 13;13:880905. doi: 10.3389/fneur.2022.880905. PMID: 35769361; PMCID: PMC9234108.
Nowinski CJ, Bureau SC, Buckland ME, Curtis MA, Daneshvar DH, Faull RLM, Grinberg LT, Hill-Yardin EL, Murray HC, Pearce AJ, Suter CM, White AJ, Finkel AM, Cantu RC. Applying the Bradford Hill Criteria for Causation to Repetitive Head Impacts and Chronic Traumatic Encephalopathy. Front Neurol. 2022 Jul 22;13:938163. doi: 10.3389/fneur.2022.938163. PMID: 35937061; PMCID: PMC9355594.
Suter CM, Affleck AJ, Lee M, Davies D, Burns AL, Sy J, I'Ons B, Buckland ME. Chronic Traumatic Encephalopathy in a Routine Neuropathology Service in Australia. J Neuropathol Exp Neurol. 2022 Sep 19;81(10):790-795. doi: 10.1093/jnen/nlac071. PMID: 35947764.
Butler MLMD, Dixon E, Stein TD, Alvarez VE, Huber B, Buckland ME, McKee AC, Cherry JD. Tau Pathology in Chronic Traumatic Encephalopathy is Primarily Neuronal. J Neuropathol Exp Neurol. 2022 Sep 19;81(10):773-780. doi: 10.1093/jnen/nlac065. Erratum in: J Neuropathol Exp Neurol. 2023 Mar 20;82(4):373. PMID: 35903039; PMCID: PMC9487650.
Watson CJG, Lawlor M, Sy J, Krishnaswamy M, Buckland ME, Brennan JW, Satgunaseelan L. Anaplasia and age of onset in desmoplastic infantile ganglioglioma: Case report and review of the literature. Pediatr Blood Cancer. 2023 Jan;70(1):e29808. doi: 10.1002/pbc.29808. Epub 2022 Jun 7. PMID: 35670752.
Suter CM, Affleck AJ, Lee M, Pearce AJ, Iles LE, Buckland ME. Chronic traumatic encephalopathy in Australia: the first three years of the Australian Sports Brain Bank. Med J Aust. 2022 Jun 6;216(10):530-531. doi: 10.5694/mja2.51420. Epub 2022 Feb 10. PMID: 35144312; PMCID: PMC9305557.
Yuile A, Satgunaseelan L, Wei J, Kastelan M, Back MF, Lee M, Wei H, Buckland ME, Lee A, Wheeler HR. Implications of Concurrent IDH1 and IDH2 Mutations on Survival in Glioma-A Case Report and Systematic Review. Curr Issues Mol Biol. 2022 Oct 21;44(10):5117-5125. doi: 10.3390/cimb44100348. PMID: 36286062; PMCID: PMC9600580.
Goire N, Edwards L, Thomas P, Bhaskar SMM, Cordato D, Buckland ME, Beran RG. Creutzfeldt-Jakob Disease in South West Sydney 2014-2020: An Unusually High Incidence of a Rare Disease. Neuroepidemiology. 2022;56(1):59-65. doi: 10.1159/000520736. Epub 2021 Nov 11. PMID: 34763340.
Lasocki A, Buckland ME, Drummond KJ, Wei H, Xie J, Christie M, Neal A, Gaillard F. Conventional MRI features can predict the molecular subtype of adult grade 2-3 intracranial diffuse gliomas. Neuroradiology. 2022 Dec;64(12):2295-2305. doi: 10.1007/s00234-022-02975-0. Epub 2022 May 24. PMID: 35606654; PMCID: PMC9643259.
Alzoubi I, Bao G, Zhang R, Loh C, Zheng Y, Cherepanoff S, Gracie G, Lee M, Kuligowski M, Alexander KL, Buckland ME, Wang X, Graeber MB. An Open-Source AI Framework for the Analysis of Single Cells in Whole-Slide Images with a Note on CD276 in Glioblastoma. Cancers (Basel). 2022 Jul 15;14(14):3441. doi: 10.3390/cancers14143441. PMID: 35884502; PMCID: PMC9316952.
Spiteri AG, Wishart CL, Pamphlett R, Locatelli G, King NJC. Microglia and monocytes in inflammatory CNS disease: integrating phenotype and function. Acta Neuropathol. 2022 Feb;143(2):179-224. doi: 10.1007/s00401-021-02384-2. Epub 2021 Dec 1. PMID: 34853891; PMCID: PMC8742818.
Spiteri AG, Wishart CL, Pamphlett R, Locatelli G, King NJC. Microglia and monocytes in inflammatory CNS disease: integrating phenotype and function. Acta Neuropathol. 2022 Feb;143(2):179-224. doi: 10.1007/s00401-021-02384-2. Epub 2021 Dec 1. PMID: 34853891; PMCID: PMC8742818.
Freydenzon A, Nabais MF, Lin T, Williams KL, Wallace L, Henders AK, Blair IP, Wray NR, Pamphlett R, McRae AF. Association between DNA methylation variability and self-reported exposure to heavy metals. Sci Rep. 2022 Jun 22;12(1):10582. doi: 10.1038/s41598-022-13892-w. PMID: 35732753; PMCID: PMC9217962.
Grima N, Henden L, Fearnley LG, Rowe DB, D'Silva S, Pamphlett R, Adams L, Kiernan MC, Mazumder S, Timmins HC, Zoing M, Bahlo M, Blair IP, Williams KL. NEK1 and STMN2 short tandem repeat lengths are not associated with Australian amyotrophic lateral sclerosis risk. Neurobiol Aging. 2022 Aug;116:92-95. doi: 10.1016/j.neurobiolaging.2022.04.012. Epub 2022 May 22. PMID: 35613520.
Restuadi R, Garton FC, Benyamin B, Lin T, Williams KL, Vinkhuyzen A, van Rheenen W, Zhu Z, Laing NG, Mather KA, Sachdev PS, Ngo ST, Steyn FJ, Wallace L, Henders AK, Visscher PM, Needham M, Mathers S, Nicholson G, Rowe DB, Henderson RD, McCombe PA, Pamphlett R, Blair IP, Wray NR, McRae AF. Polygenic risk score analysis for amyotrophic lateral sclerosis leveraging cognitive performance, educational attainment and schizophrenia. Eur J Hum Genet. 2022 May;30(5):532-539. doi: 10.1038/s41431-021-00885-y. Epub 2021 Apr 27. PMID: 33907316; PMCID: PMC9090723.
van Rheenen W, van der Spek RAA, Bakker MK, van Vugt JJFA, Hop PJ, Zwamborn RAJ, de Klein N, Westra HJ, Bakker OB, Deelen P, Shireby G, Hannon E, Moisse M, Baird D, Restuadi R, Dolzhenko E, Dekker AM, Gawor K, Westeneng HJ, Tazelaar GHP, van Eijk KR, Kooyman M, Byrne RP, Doherty M, Heverin M, Al Khleifat A, Iacoangeli A, Shatunov A, Ticozzi N, Cooper-Knock J, Smith BN, Gromicho M, Chandran S, Pal S, Morrison KE, Shaw PJ, Hardy J, Orrell RW, Sendtner M, Meyer T, Başak N, van der Kooi AJ, Ratti A, Fogh I, Gellera C, Lauria G, Corti S, Cereda C, Sproviero D, D'Alfonso S, Sorarù G, Siciliano G, Filosto M, Padovani A, Chiò A, Calvo A, Moglia C, Brunetti M, Canosa A, Grassano M, Beghi E, Pupillo E, Logroscino G, Nefussy B, Osmanovic A, Nordin A, Lerner Y, Zabari M, Gotkine M, Baloh RH, Bell S, Vourc'h P, Corcia P, Couratier P, Millecamps S, Meininger V, Salachas F, Mora Pardina JS, Assialioui A, Rojas-García R, Dion PA, Ross JP, Ludolph AC, Weishaupt JH, Brenner D, Freischmidt A, Bensimon G, Brice A, Durr A, Payan CAM, Saker-Delye S, Wood NW, Topp S, Rademakers R, Tittmann L, Lieb W, Franke A, Ripke S, Braun A, Kraft J, Whiteman DC, Olsen CM, Uitterlinden AG, Hofman A, Rietschel M, Cichon S, Nöthen MM, Amouyel P; SLALOM Consortium; PARALS Consortium; SLAGEN Consortium; SLAP Consortium; Traynor BJ, Singleton AB, Mitne Neto M, Cauchi RJ, Ophoff RA, Wiedau-Pazos M, Lomen-Hoerth C, van Deerlin VM, Grosskreutz J, Roediger A, Gaur N, Jörk A, Barthel T, Theele E, Ilse B, Stubendorff B, Witte OW, Steinbach R, Hübner CA, Graff C, Brylev L, Fominykh V, Demeshonok V, Ataulina A, Rogelj B, Koritnik B, Zidar J, Ravnik-Glavač M, Glavač D, Stević Z, Drory V, Povedano M, Blair IP, Kiernan MC, Benyamin B, Henderson RD, Furlong S, Mathers S, McCombe PA, Needham M, Ngo ST, Nicholson GA, Pamphlett R, Rowe DB, Steyn FJ, Williams KL, Mather KA, Sachdev PS, Henders AK, Wallace L, de Carvalho M, Pinto S, Petri S, Weber M, Rouleau GA, Silani V, Curtis CJ, Breen G, Glass JD, Brown RH Jr, Landers JE, Shaw CE, Andersen PM, Groen EJN, van Es MA, Pasterkamp RJ, Fan D, Garton FC, McRae AF, Davey Smith G, Gaunt TR, Eberle MA, Mill J, McLaughlin RL, Hardiman O, Kenna KP, Wray NR, Tsai E, Runz H, Franke L, Al-Chalabi A, Van Damme P, van den Berg LH, Veldink JH. Author Correction: Common and rare variant association analyses in amyotrophic lateral sclerosis identify 15 risk loci with distinct genetic architectures and neuron-specific biology. Nat Genet. 2022 Mar;54(3):361. doi: 10.1038/s41588-022-01020-3. Erratum for: Nat Genet. 2021 Dec;53(12):1636-1648. PMID: 35102318; PMCID: PMC8920884.
Pamphlett R, Bishop DP. Mercury is present in neurons and oligodendrocytes in regions of the brain affected by Parkinson's disease and co-localises with Lewy bodies. PLoS One. 2022 Jan 11;17(1):e0262464. doi: 10.1371/journal.pone.0262464. PMID: 35015796; PMCID: PMC8752015.
Luu W, Sy J, Luu Q. Necrotising autoimmune myopathy: a differential diagnosis for deranged liver function tests. Med J Aust. 2022 Jul 4;217(1):20-22. doi: 10.5694/mja2.51609. Epub 2022 Jun 13. PMID: 35698437.
Yuile A, Khasraw M, Low JT, Walsh KM, Lipp E, Sy J, Satgunaseelan L, Kastelan MA, De Silva M, Lee A, Wheeler H. Patterns of care in adult histone mutant gliomas: Results of an international survey. Neurooncol Pract. 2022 Jun 5;9(6):520-525. doi: 10.1093/nop/npac047. PMID: 36388418; PMCID: PMC9665055.
Horak P, Griffith M, Danos AM, Pitel BA, Madhavan S, Liu X, Chow C, Williams H, Carmody L, Barrow-Laing L, Rieke D, Kreutzfeldt S, Stenzinger A, Tamborero D, Benary M, Rajagopal PS, Ida CM, Lesmana H, Satgunaseelan L, Merker JD, Tolstorukov MY, Campregher PV, Warner JL, Rao S, Natesan M, Shen H, Venstrom J, Roy S, Tao K, Kanagal-Shamanna R, Xu X, Ritter DI, Pagel K, Krysiak K, Dubuc A, Akkari YM, Li XS, Lee J, King I, Raca G, Wagner AH, Li MM, Plon SE, Kulkarni S, Griffith OL, Chakravarty D, Sonkin D. Standards for the classification of pathogenicity of somatic variants in cancer (oncogenicity): Joint recommendations of Clinical Genome Resource (ClinGen), Cancer Genomics Consortium (CGC), and Variant Interpretation for Cancer Consortium (VICC). Genet Med. 2022 May;24(5):986-998. doi: 10.1016/j.gim.2022.01.001. Epub 2022 Jan 29. Erratum in: Genet Med. 2022 Sep;24(9):1991. PMID: 35101336; PMCID: PMC9081216.
Fielder T, Butler J, Tierney G, Holmes M, Lam KY, Satgunaseelan L, Colebatch AJ, Mahar A, Gupta R, O'Toole S, Cooper WA. ROS1 rearrangements in lung adenocarcinomas are defined by diffuse strong immunohistochemical expression of ROS1. Pathology. 2022 Jun;54(4):399-403. doi: 10.1016/j.pathol.2021.07.012. Epub 2021 Oct 25. PMID: 34702583.
Gorolay V, Martens D, Satgunaseelan L, Van Camp L, Winkler G. Hemorrhagic Meningitis Due to Varicella-Zoster Virus: A Case Report With Clinical, Cytologic, and Radiologic Correlation. Cureus. 2022 Nov 5;14(11):e31113. doi: 10.7759/cureus.31113. PMID: 36479400; PMCID: PMC9720349.
Horak P, Griffith M, Danos AM, Pitel BA, Madhavan S, Liu X, Chow C, Williams H, Carmody L, Barrow-Laing L, Rieke D, Kreutzfeldt S, Stenzinger A, Tamborero D, Benary M, Rajagopal PS, Ida CM, Lesmana H, Satgunaseelan L, Merker JD, Tolstorukov MY, Campregher PV, Warner JL, Rao S, Natesan M, Shen H, Venstrom J, Roy S, Tao K, Kanagal-Shamanna R, Xu X, Ritter DI, Pagel K, Krysiak K, Dubuc A, Akkari YM, Li XS, Lee J, King I, Raca G, Wagner AH, Li MM, Plon SE, Kulkarni S, Griffith OL, Chakravarty D, Sonkin D. Standards for the classification of pathogenicity of somatic variants in cancer (oncogenicity): Joint recommendations of Clinical Genome Resource (ClinGen), Cancer Genomics Consortium (CGC), and Variant Interpretation for Cancer Consortium (VICC). Genet Med. 2022 Sep;24(9):1991. doi: 10.1016/j.gim.2022.07.001. Erratum for: Genet Med. 2022 May;24(5):986-998. PMID: 36063163.
Satgunaseelan L, Strbenac D, Tadi S, Nguyen K, Wykes J, Palme CE, Low TH, Yang JYH, Clark JR, Gupta R. Viral Integration Plays a Minor Role in the Development and Prognostication of Oral Squamous Cell Carcinoma. Cancers (Basel). 2022 Oct 24;14(21):5213. doi: 10.3390/cancers14215213. PMID: 36358632; PMCID: PMC9656962.
Jackett LA, Satgunaseelan L, Roper E, Lo SN, Thompson JF, Scolyer RA. Residual melanoma in wide local excision specimens after 'complete' excision of primary cutaneous in situ and invasive melanomas. Pathology. 2022 Feb;54(1):71-78. doi: 10.1016/j.pathol.2021.05.094. Epub 2021 Aug 13. PMID: 34392983.
Satgunaseelan L, Strbenac D, Willet C, Chew T, Sadsad R, Wykes J, Low TH, Cooper WA, Lee CS, Palme CE, Yang JYH, Clark JR, Gupta R. Whole genome duplication in oral squamous cell carcinoma in patients younger than 50 years: implications for prognosis and adverse clinicopathological factors. Genes Chromosomes Cancer. 2022 Sep;61(9):561-571. doi: 10.1002/gcc.23076. Epub 2022 Jun 24. PMID: 35670448; PMCID: PMC9542139.
Yabe TE, King K, Russell S, Satgunaseelan L, Gupta R, Chen J, Ashford B. "MYH9 mutation and squamous cell cancer of the tongue in a young adult: a novel case report". Diagn Pathol. 2022 Feb 6;17(1):23. doi: 10.1186/s13000-022-01210-x. PMID: 35125114; PMCID: PMC8818095.
Griffin CP, Paul CL, Alexander KL, Walker MM, Hondermarck H, Lynam J. Postmortem brain donations vs premortem surgical resections for glioblastoma research: viewing the matter as a whole. Neurooncol Adv. 2021 Nov 18;4(1):vdab168. doi: 10.1093/noajnl/vdab168. PMID: 35047819; PMCID: PMC8760897.
Reher R, Aron AT, Fajtová P, Stincone P, Wagner B, Pérez-Lorente AI, Liu C, Shalom IYB, Bittremieux W, Wang M, Jeong K, Matos-Hernandez ML, Alexander KL, Caro-Diaz EJ, Naman CB, Scanlan JHW, Hochban PMM, Diederich WE, Molina-Santiago C, Romero D, Selim KA, Sass P, Brötz-Oesterhelt H, Hughes CC, Dorrestein PC, O'Donoghue AJ, Gerwick WH, Petras D. Native metabolomics identifies the rivulariapeptolide family of protease inhibitors. Nat Commun. 2022 Aug 8;13(1):4619. doi: 10.1038/s41467-022-32016-6. PMID: 35941113; PMCID: PMC9358669.